ISO 18209-1:2024
Biotechnology — Biobanking of parasites — Part 1: Helminths

Standard No.

ISO 18209-1:2024

Release Date

2024

Published By

International Organization for Standardization (ISO)  IX  /  ISO

Latest

ISO 18209-1:2024

Introduction

Analysis of the core content of the standard

Scope of application and definition of terms

This standard applies to all biological sample repositories engaged in worm research, and clearly defines the configuration requirements for key equipment such as biosafety workstations and ultra-low temperature storage equipment.

• Biobank: a legal entity that maintains a collection of parasites
• Hermes: multicellular invertebrate parasites (nematodes, tapeworms, etc.)
• Minimum Data Set (MDS): basic data fields required for specimen identification

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Biosafety Management System

Biosafety Level	Facility Requirements	Typical Parasites
Level 1	Basic laboratory partitions with hand washing sinks	Non-pathogenic helminths
Level 2	Automatic closing buffer room, emergency eyewash device	Ascaris, hookworm
Level 3	One-way airflow system, sealed penetration	Schistosome

According to the requirements of the 4th edition of the WHO guidelines, sample processing must be equipped with a biosafety cabinet of the corresponding level, and wastewater must be specially treated to eliminate the risk of worm egg contamination.

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Sample life cycle management

Key process nodes:

1. Collection: adults (host organs/feces), larvae (muscle/blood), eggs (urine/sputum)
2. Pretreatment: formalin fixation (morphology), ≥70% ethanol storage (molecular research)
3. Identification: morphological examination combined with DNA sequencing
4. Storage: liquid nitrogen (≤-140℃), ultra-low temperature (≤-80℃) dual backup storage

Typical application case:

Worm samples for the treatment of Crohn's disease must meet the following requirements: ① Morphological integrity (body length variation ≤5%); ② DNA integrity (OD260/280=1.8-2.0); ③ Pathogen detection is negative.

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Key indicators of quality control

Sample type	Detection method	Qualification standard
Immersion sample	DNA sequencing contamination test	No exogenous sequence
Slide sample	Morphological integrity check	Structural integrity rate ≥95%
RNA sample	UV absorption test	OD260/280≥2.0

Implementation Suggestions

• Equipment configuration: Dual compressor ultra-low temperature refrigerator must be equipped with a temperature alarm system, and an oxygen monitoring device must be installed in the liquid nitrogen storage area
• Staff training: Insect egg identification requires ≥50 hours of microscope operation experience
• Document management: Use ISO 8601-1 standard to record timestamps to ensure audit traceability