GB/T 43641-2024
Technical specifications for biological full sibling relationship identification (English Version)

Standard No.

GB/T 43641-2024

Language

Chinese, Available in English version

Release Date

2024

Published By

General Administration of Quality Supervision, Inspection and Quarantine of the People‘s Republic of China  CN  /  GB

Latest

GB/T 43641-2024

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Introduction

Interpretation of the National Standard of the People's Republic of China GB/T 43641—2024

I. Background and Significance of Standard Formulation

The release of the Technical Specification for the Identification of Biological Full Sibling Relationships (GB/T 43641—2024) fills the gap in the identification of full sibling relationships in the field of domestic forensic evidence. With the advancement of DNA testing technology, especially the application of STR locus typing technology, the demand for the determination of full sibling relationships is increasing.

II. Standard Core Content Framework

Chapters	Core Content	Keywords
Terms and Definitions	Full siblings, full sibling relationship identification, and consistency score of status (IBS).	- Full siblings - IBS score
Test procedures	Including sample collection, DNA extraction and purification, PCR amplification and typing, etc.	- DNA extraction - PCR amplification
Parameter calculation	Calculation method of CIBS (Cumulative Consistency of State Score) and CFSI (Cumulative Full Sibling Index).	- CIBS - CFSI
Decision threshold	Thresholds and system performance of CIBS and CFSI under different loci detection systems.	- Decision threshold - System performance

III. Implementation suggestions and precautions

1. Optimization suggestions for the detection system

• It is recommended to use a detection system that includes 19 mandatory loci as the basis.
• Add STR loci that are not in linkage disequilibrium with the required loci according to actual needs to improve detection efficiency.
• It is recommended to give priority to STR loci with individual identification ability ≥ 0.9000 to ensure the reliability of the identification results.

2. Precautions for sample collection and processing

• It is recommended to select blood, saliva, oral swabs, etc. as sample types, and avoid using blood samples after peripheral blood stem cell transplantation.
• Strictly follow the requirements of GA/T 1162 for sample collection, packaging and storage to ensure the integrity and purity of DNA.

GB/T 43641-2024 Referenced Document

• GA/T 1162 Specifications for collection, preservation and delivery of forensic biologic samples
• GA/T 1163 Analysis and application of human DNA fluorescent STR typing results
• SF/T 0069 Forensic Evidence Identification Laboratory Management Standards
• SF/T 0134 Technical specifications for nucleic acid extraction from forensic biological test materials

GB/T 43641-2024 history

• 2024 GB/T 43641-2024 Technical specifications for biological full sibling relationship identification

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